|
KMID : 0363020090390030359
|
|
Journal of Korean Academy of Periodontology
2009 Volume.39 No. 3 p.359 ~ p.365
|
|
|
Response of fetal rat calvarial cells on mineral trioxide aggregate after IL-1β stimulation
|
|
Lee Sul-Hyun
Park Ji-Il
Kim Young-Joon
|
|
|
Abstract
|
|
|
Purpose: The purpose of this study was to investigate the ability of Mineral trioxide aggregate(MTA) to support osteoclastic differentiation from fetal rat calvarial cell.
Methods: In this study, response of IL-6, RANKL, and OPG in fetal rat calvarial cells stimulated with IL-1β on MTA was evaluated by ELISA and RT-PCR.
Results: The results were as follows; there was no significant difference between glass and MTA at 5days. In ELISA analysis, Glass group and MTA group showed similar IL-6 expression, Glass+IL-1β group and MTA+IL-1β group showed similar IL-6 expression. In RT-PCR analysis, Glass group and MTA group showed similar IL-6, RANKL, OPG mRNA expression, MTA+IL-1β group and Glass+IL-1β group showed 3 fold increase of IL-6 and RNAKL mRNA expression when compared with MTA group. All groups showed similar OPG mRNA expression.
Conclusions: MTA does not suppress cell proliferation and increase the proinflammatory cytokine that induce osteoclastogenesis. Thus, MTA is biocompatible material that could be used in various clinical conditions.
|
|
|
KEYWORD
|
|
|
biocompatible materials, cytokines, ELISA, mineral trioxide aggregate
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|